Renal cell carcinoma. Immunohistological study to the expression of the inactive form of the pyruvatekinase

Purpose: The inactive form of pyruvatekinase could be established in differ ent tumours. Purpose of this study was to demonstate the presence or absenc e of the inactive form of pyruvatekinase in renal cell carcinoma, metastase s and benign renal tissue by immunohistology. Method: After deparaffinization of formaline-fixed tissue (5 original tumou rs, 2 metastases and 5 benign renal tissues) cells were stained (APAAP-meth od) with Cion DF4 (ScheBo Tech). Results: All malign tissue showed a positive reactin, inside benign tissue we saw a positive reaction of endothelial cells however we saw no reaktion with benign renal cells. Conclusion: Renal cell carcinoma and metastatic cells show a strong immunoh istological reaction against the inactive form of pyruvatekinase, no reacti on of benign renal cells. It should be possible to develope a serological t umourmarker for renal cell carcinoma.