CLONING AND SEQUENCE-ANALYSIS OF A CLASS-A BETA-LACTAMASE FROM MYCOBACTERIUM-TUBERCULOSIS H37RA

Authors
HACKBARTH CJ UNSAL I CHAMBERS HF
Citation
Cj. Hackbarth et al., CLONING AND SEQUENCE-ANALYSIS OF A CLASS-A BETA-LACTAMASE FROM MYCOBACTERIUM-TUBERCULOSIS H37RA, Antimicrobial agents and chemotherapy, 41(5), 1997, pp. 1182-1185
Citations number
31
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
41
Issue
5
Year of publication
1997
Pages
1182 - 1185
Database
ISI
SICI code
0066-4804(1997)41:5<1182:CASOAC>2.0.ZU;2-9
Abstract
A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increa sed resistance to cefoxitin were identified. Isoelectric focusing dete cted an M. tuberculosis-derived beta-lactamase in one of these recombi nant clones. A sequence analysis identified it as a class A beta-lacta mase whose expression correlated with the increased resistance phenoty pe.