CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF RABBIT ALPHA-1A-ADRENOCEPTOR

Authors
MIYAMOTO S TANIGUCHI T SUZUKI F TAKITA M KOSAKA N NEGORO E OKUDA T KOSAKA H MURATA S NAKAMURA S AKAGI Y OSHITA M WATANABE Y MURAMATSU I
Citation
S. Miyamoto et al., CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF RABBIT ALPHA-1A-ADRENOCEPTOR, Life sciences, 60(23), 1997, pp. 2069-2074
Citations number
22
Categorie Soggetti
Biology,"Medicine, Research & Experimental","Pharmacology & Pharmacy
Journal title
Life sciences → ACNP
ISSN journal
00243205
Volume
60
Issue
23
Year of publication
1997
Pages
2069 - 2074
Database
ISI
SICI code
0024-3205(1997)60:23<2069:CFEATD>2.0.ZU;2-3
Abstract
A cDNA clone, which has an open reading frame of 1398 nucleotides enco ding a 466-amino-acid peptide, has been isolated from rabbit liver cDN A library. Compared with the peptide sequence, it shows high homology to ala adrenoceptors of human, bovine and rat. We expressed this clone in COS-7 and investigated the pharmacological properties, revealing s imilarity to those of human ala adrenoceptors. Competitive RT/PCR has detected the mRNA in variety of rabbit tissues, especially abundantly in liver, vas deferens, brain, and aorta, but not in heart.