Estrogen receptor-positive proliferating cells in the normal and precancerous breast

Recently it has been shown that epithelial cell expression of the estrogen receptor CER) and that of the proliferation-associated marker Ki-67 are alm ost mutually exclusive in the normal premenopausal human breast but that co expression frequently occurs in estrogen receptor-positive (ER+) breast can cers. This coexpression may indicate disordered expression of ER in the cel l cycle or failure to suppress division of ER+ cells and could be important in neoplastic transformation. The purpose of this study was to determine w hether in situ proliferations known to be associated with different levels of risk for developing breast cancer contain these coexpressing cells and, if so, the stage at which they occur. We found that ER+ proliferating cells were rare in premenopausal lobules but increased with age in the normal br east. There was no difference in nonlesional tissue between cancerous and n oncancerous breasts. The percentage of dual-expressing cells was significan tly increased, however, in all of the in situ proliferations and correlated positively with the level of risk of developing breast cancer. We suggest that development of at least some human breast cancers is associated with i ncreasing failure to down-regulate ER as cells enter the cycle or to suppre ss division of ER+ cells. The mechanism may involve the loss of a tumor sup presser gene.