M. Mak et L. Balaspiri, Structure identification of synthetic human galanin using tandem mass spectrometry and in situ fast atom bombardment mapping, ANALYT CHIM, 402(1-2), 1999, pp. 303-309
For the primary structure analysis of synthetic human galanin and several o
f its segments, the combination of the fast atom bombardment mapping and th
e tandem mass spectrometric method was applied. Multistep enzymic digestion
performed in situ, i.e. directly on the probe tip and the subsequent high
and low energy collision induced dissociation of the resulting hydrolytic p
roducts led to sufficient sequence information to elucidate and confirm the
structure of the synthetic galanin derivatives. (C) 1999 Elsevier Science
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