Structure identification of synthetic human galanin using tandem mass spectrometry and in situ fast atom bombardment mapping

For the primary structure analysis of synthetic human galanin and several o f its segments, the combination of the fast atom bombardment mapping and th e tandem mass spectrometric method was applied. Multistep enzymic digestion performed in situ, i.e. directly on the probe tip and the subsequent high and low energy collision induced dissociation of the resulting hydrolytic p roducts led to sufficient sequence information to elucidate and confirm the structure of the synthetic galanin derivatives. (C) 1999 Elsevier Science B.V. All rights reserved.