Autoantibodies against P53 protein in patients with transitional cell carcinoma of the bladder

Cellular accumulation ann conformational changes of mutant p53 could act as immunogens for, auto-antibodies (auto-Abs) generation when altered p53 fro m tumoral cells reaches the blood stream. Out main objective was to compare the presence and clinical implications of p53-antibodies in serum with the immunohistochemical (IHC) tissue overexpression of protein p53 in patients with transitional cell carcinoma of the bladder, evaluating their associat ion with bladder cancer parameters ann their prognostic value. The study co mprised 59 patients with bladder cancel (group 1) and 15 healthy controls ( group 2). Serum p53-Abs were measured by ELISA. Mutant p53 protein IHC over expression was examined front paraffin embedded tissues using monoclonal DO -7 Ab. Serum 1 p53-Abs were detected in 14/59 and IHC P53 was positive in 2 4/59 patients from group 1. All p53-Abs positive patients had IHC p53 posit ive tumors, but some patients with IHC positive immunoreactivity showed und etectable p53-Abs. None of the healthy controls had detectable p53-Abs. Tit res of p53-Abs were associated with stage and grade. P53 overexpression was dependant on stage, grade, pattern of growth and focality. P53 Abs showed a significant prognostic value for disease free survival (p = 0.0059) and l ife expectancy (p < 0.0005) and for IHQ p53 for. life expectancy (p = 0.003 3). Patients with positive P53 Abs showed a higher probability for a shorte r survival OR = 6.38 (1.77-22.99) than those who were positive for IHQ p53 OR = 4.00 (1.31-12.8) or those who were negative for p53 Abs and/or IHQ p53 . The measurement of p53 Abs in serum appeared to be a simple determination which might reflect the p53 status and might help in the selection of thos e bladder cancer patients with a worse prognosis.