Lk. Ng et al., Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar typhimurium DT104, ANTIM AG CH, 43(12), 1999, pp. 3018-3021
PCR was used to identify antibiotic resistance determinants in 31 Canadian
Salmonella serovar Typhimurium DT104 isolates. Genes encoding resistance to
ampicillin (pse1 or blaP1), chloramphenicol (pasppflo-like), streptomycin-
spectinomycin (aadA2), sulfonamide (sulI), and tetracycline [tet(G)] Here m
apped to a 13-kb region of DNA of one isolate. Two copies of sulI were iden
tified and mapped to the 3' end of either pse1 or aadA2 integrons. The two
integrons were separated by the pasppflo-like gene and the tet(G) gene. The
kanamycin resistance determinant (aphA-1) was present on a 2.0-MDa plasmid
(five isolates) or on the chromosome (three isolates).