Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus

Authors
Zhou, R Linz, JE
Citation
R. Zhou et Je. Linz, Enzymatic function of the nor-1 protein in aflatoxin biosynthesis in Aspergillus parasiticus, APPL ENVIR, 65(12), 1999, pp. 5639-5641
Citations number
13
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
12
Year of publication
1999
Pages
5639 - 5641
Database
ISI
SICI code
0099-2240(199912)65:12<5639:EFOTNP>2.0.ZU;2-6
Abstract
The nor-1 gene is involved in aflatoxin biosynthesis in Aspergillus parasit icus and was predicted to encode a norsolorinic acid ketoreductase. Recombi nant Nor-1 expressed in Escherichia coli converted the 1' keto group of nor solorinic acid to the 1' hydroxyl group of averantin in crude E. coli cell extracts in the presence of NADPH. The results confirm that Nor-1 functions as a ketoreductase in vitro.