An extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial waste water

A bacterial biofilm, capable of mineralising a technical mixture of cis- an d tr trans-1,3-dichloropropene (DCPE), was enriched on the biomedium side o f an extractive membrane biofilm reactor (EMBR). The membrane separates the biomedium from the industrial waste water, in terms of pH, ionic strength and the concentration of toxic chemicals. The biofilm, attached to a silico ne membrane? is able to mineralise DCPE after its diffusion through the mem brane. Five bacterial strains with degradation capabilities were isolated f rom the metabolically active biofilm and further investigated in batch expe riments. Two of them, Rhodococcus erythropolis strains EK2 and EK5, can gro w with DCPE as the sole carbon source. Pseudomonas sp. EK1 utilises cis-3-c hloroallylalcohol and cis-3-chloroacrylic acid, whereas the metabolite tran s-3-chloroacrylic acid represents a dead-end product of the pathway of this strain. The other two strains, Delftia sp. EK3 and EK4, although unable to grow with DCPE as the carbon source, can transform DCPE and its upper-path way intermediates at reasonable conversion rates. They may represent helper functions of the biofilm consortium, which mineralised up to 12.5 mmol DCP E per hour per gram of biomass protein. Higher feed rates in the EMBR (up t o 15 mmol per hour per 100-1 bioreactor volume) and shock loads correspondi ng to concentrations up to 1.8 mmol l(-1) led to a significant increase in the freely floating bacterial biomass in the reactor medium (OD546 = 0.2) A t the standard operating feed rate of 1.8 mmol h(-1), the free biomass conc entration was very low (OD546 = 0.04).