IMMUNODETECTION OF A PROTEIN RELATED TO MAMMALIAN ARRESTIN IN EUGLENA-GRACILIS

Authors
RAZAGHI A BONALY J CHACUN H FAURE JP MIRSHAHI M BARQUE JP
Citation
A. Razaghi et al., IMMUNODETECTION OF A PROTEIN RELATED TO MAMMALIAN ARRESTIN IN EUGLENA-GRACILIS, Biochemical and biophysical research communications, 233(3), 1997, pp. 601-605
Citations number
16
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
233
Issue
3
Year of publication
1997
Pages
601 - 605
Database
ISI
SICI code
0006-291X(1997)233:3<601:IOAPRT>2.0.ZU;2-H
Abstract
Six monoclonal antibodies directed against different epitopes of bovin e retinal arrestin recognized a single polypeptide in extracts of achl orophyllous Euglena gracilis cells. This polypeptide had an apparent m olecular weight slightly lower: 45kDa vs. 48 kDa, and a more basic iso electric point compared to that of bovine visual arrestin. It was loca ted in an insoluble cytoplasmic fraction. Immunofluorescence assays sh ow a cytoplasmic punctuated pattern suggesting a cluster distribution or a linkage to some cell structure. The presence of arrestine-like mo lecules in achlorophyllous Euglena gracilis cells suggests that these proteins might be involved in a peculiar step of chemical signal trans duction processes.