Hg. Jeong et al., MURINE CYP1A-1 INDUCTION IN MOUSE HEPATOMA HEPA-1C1C7 CELLS BY MYRISTICIN, Biochemical and biophysical research communications, 233(3), 1997, pp. 619-622
Mouse hepatoma Hepa-1c1c7 (Hepa-l) cells were treated with myristicin
to assess the role of myristicin in the process of Cyp1a-1 induction.
Treatment of Hepa-l cells with myristicin increased Cyp1a-1 transcript
ion in a dose-dependent manner as shown by analysis of 7-ethoxyresoruf
in O-deethylase activity, Cypla-1 protein level, and Cypla-1 mRNA. Myr
isticin, however, did not competitively displace [H-3]2,3,7,8-tetrachl
orodibenzo-p-dioxin from the Hepa-l cytosolic aryl hydrocarbon (Ah) re
ceptor in a competitive Ah receptor binding analysis using sucrose den
sity gradient sedimentation and did not affect formation of DNA-protei
n complexes between the Ah receptor and its DRE target in a gel mobili
ty shift assay using oligonucleotides corresponding to DRE 3 of the Cy
p1a-1. These results suggest that the induction of Cyp1a-1 gene expres
sion by myristicin in Hepa-l cells might occur through an Ah receptor-
independent pathway. (C) 1997 Academic Press.