SUPPRESSION OF NEUTRAL CHOLESTEROL ESTER HYDROLASE ACTIVITY BY ANTISENSE DNA OF HORMONE-SENSITIVE LIPASE

In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) fr om a molecular perspective, Chinese hamster ovary (CHO) cells were tra nsfected with rat antisense hormone-sensitive lipase cDNA, and three d ifferent cell lines, designated as anti-HSL, were established. NCEH ac tivity in anti-HSL cells was reduced to approximately 50% of that in c ontrol CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a fun ction Of NCEH as well. (C) 1997 Academic Press.