S. Ramani et Sk. Apte, TRANSIENT EXPRESSION OF MULTIPLE GENES IN SALINITY-STRESSED YOUNG SEEDLINGS OF RICE (ORYZA-SATIVA L) CV BURA-RATA, Biochemical and biophysical research communications, 233(3), 1997, pp. 663-667
To date only about 15 abundantly expressed osmoresponsive genes/protei
ns have been described in rice cultivars. Using in vivo radiolabeling
followed by two-dimensional electrophoresis and autoradiography, a rec
ord number of at least 35 salinity stress-induced polypeptides (14-90
kDa) and 17 salt stress-repressed polypeptides were detected in a halo
tolerant Indica rice cultivar Bura Rata. These include >20 hitherto un
reported rice polypeptides that exhibited a low abundance, short-term
expression during NaCl stress. Prolonged exposure to NaCl decreased th
eir synthesis. These findings have widened the scope of further invest
igations into new osmoresponsive genes, especially those with possibly
transient regulatory functions in rice halotolerance. (C) 1997 Academ
ic Press.