Mice lacking Pin1 develop normally, but are defective in entering cell cycle from G(0) arrest

The peptidyl prolil cis/trans isomerase Ess1/Pin1 is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. To investigate the function of Pin1 in mammalian cells, we created mice lacking Pin1. These mice underwent normal development. Althou gh the embryonic Pin1-/- fibroblasts grew normally, they proved significant ly deficient in their ability to restart proliferation in response to serum stimulation after G(0) arrest. These results suggest that Pin1 is required for cell cycle progression from G(0) arrest as well as mitosis progression in normal mammalian cells. (C) 1999 Academic Press.