Constitutive secretion of an endogenous insulin-like peptide binding protein with high affinity for insulin in Spodoptera frugiperda (Sf9) cell cultures

Serum-free medium from batch cultures of Sf9 insect cells was examined for the occurrence of proteins related to the insulin-like growth factor family . We found that the Sf9 cell line constitutively produced and secreted a so luble protein with a MW of 27 kDa that exerted specific binding to human in sulin-like growth factor-I (IGF-I) and -II. Moreover, the secreted protein bound human insulin and human proinsulin with higher affinity than IGF-I an d -II. The order of affinity to the insulin peptides, determined by competi tive inhibition of ligand binding, was: insulin > proinsulin > IGF-I much g reater than IGF-II. The dissociation constant (k(d)) for IGF-II was 28.5 +/ - 1.7 nM and for insulin 7.2 +/- 1.3 nM, as determined by Scatchard plot an alysis. The results suggest that the Sf9 cells produce an insulin binding p rotein similar to the human insulin-like growth factor binding protein-rela ted protein 1 (IGFBP-rP1). (C) 1999 Academic Press.