T. Ueda et al., Novel exons located more than 200 kb downstream of the previously described 3 ' exon of the K-sam gene for generating activated forms of KGF receptor, BIOC BIOP R, 265(3), 1999, pp. 739-745
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
The K-sam gene was first identified as an amplified gene in the poorly diff
erentiated types, especially in the scirrhous type, of gastric cancers. We
have recently found and reported that the carboxyl-terminal exons of R-sam
are frequently deleted in the scirrhous type of gastric cancer. The deletio
n generates preferential expression of at least six novel K-sam-II mRNAs: K
-sam-IIH1, -IIH2 and -IIH3/O4, and K-sam-IIO1, -IIO2, and -IIO3, which enco
de novel proteins lacking the transformation-inhibitory sequence or activat
ed K-sam proteins. In this study, we investigated expression of the previou
sly described K-sam-IIC1 and -IIC3 mRNAs and the novel six K-sam-II mRNAs i
n 14 gastric cancer cell lines, 7 breast cancer cell lines, and 20 human no
rmal tissues. All the six novel K-sam-II mRNAs were expressed preferentiall
y in the cell lines derived from the scirrhous type of gastric cancers but
not in the 7 breast cancer cell lines and the 20 human normal tissues. We f
urther determined the positional relationship of four exons of H1, O1, O2,
and O3 out of the six exons of H1, H2, H3/O4, O1, O2, and O3, and found tha
t these four novel K-sam exons were located more than 200 kb downstream of
the previously described carboxyl-terminal exon of the R-sam gene. Expressi
on of K-sam-IIH1, -IIO1, and -IIO2 mRNAs encoding activated K-sam products
in the scirrhous type of gastric cancer cell lines HSC39, OCUM2M, HSC59, an
d HSC6o was not due to the deletion of the C1 exon of R-sam. (C) 1999 Acade
mic Press.