Mechanism of inhibition of cathepsin K by potent, selective 1,5-diacylcarbohydrazides: A new class of mechanism-based inhibitors of thiol proteases

The nature of the inhibition of thiol proteases by a new class of mechanism -based inhibitors, 1,5-diacylcarbohydrazides, is described. These potent, t ime-dependent, active-site spanning inhibitors include compounds that are s elective for cathepsin K, a cysteine protease unique to osteoclasts. The 1, 5- diacylcarbohydrazides are slow substrates for members of the papain supe rfamily with inhibition resulting from slow enzyme decarbamylation. Enzyme- catalyzed hydrolysis of 2,2'-N,N'-bis (benzyloxycarbonyl)-L-leucinylcarbohy drazide is accompanied by formation of a hydrazide-containing product and a carbamyl-enzyme intermediate that is sufficiently stable to be observed by mass spectrometry and NMR. Stopped-flow studies yield a saturation limited value of 43 s(-1) for the rate of cathepsin K acylation by 2,2'-N,N'-bis ( benzyloxycarbonyl)-L-leucinylcarbohydrazide. Inhibition potency varies amon g pro teases tested as reflected by 2-3 orders of magnitude differences in K-i and k(obs)/I, but all eventually form the same stable covalent intermed iate. Reactivation rates are equivalent for all enzymes tested (1 x 10(-4) s(-1)), indicating hydrolysis of a common carbamyl-enzyme form. NMR spectro scopic studies with cathepsin K and 2,2'-N,N'-bis(benzyloxycarbonyl)-L-leuc inylcarbohydrazide provide evidence of inhibitor cleavage to generate a cov alent carbamyl-enzyme intermediate rather than a tetrahedral complex. The p roduct Cbz-Leuhydrazide does not appear enzyme-bound after cleavage in the NMR spectra, suggesting that the stable inhibited form of the enzyme is the thioester complex. I,5-Diacylcarbohydrazides represent a new class of unre active cysteine protease inhibitors that share a common mechanism of action across members of the papain superfamily. Both S and S' subsite interactio ns are exploited in achieving high selectivity and potency.