Synthesis and biological activity of polyethylene glycol-mouse nerve growth factor conjugate

Conjugation of poly(ethylene glycol) derivatives with therapeutic proteins is a promising approach for enhancing protein stability and, therefore, eff ectiveness. An N-hydroxysuccinimidyl ester of fluorescein-PEG 2000 was used for chemical modification of mouse nerve growth factor (mNGF), a dimeric p rotein with therapeutic potential for Alzheimer's disease. The mNGF-PEG2000 -fluorescein conjugate was characterized by RP-HPLC, spectrofluorometry, an d SDS-PAGE and was biologically active, as determined by two independent NG F-specific assays (enhancement of ChAT activity in fetal neurons and neurit e outgrowth in PC12 cells). The conjugate was not detectable by a standard NGF ELISA, suggesting a fortuitous reduction in protein recognition by anti bodies.