Polymerization of diaspirin cross-linked hemoglobin (DCLHb) with water-soluble, nonimmunogenic polyamide cross-linking agents

Authors
Hai, TT Pereira, DE Nelson, DJ Srnak, A Catarello, J
Citation
Tt. Hai et al., Polymerization of diaspirin cross-linked hemoglobin (DCLHb) with water-soluble, nonimmunogenic polyamide cross-linking agents, BIOCONJ CHE, 10(6), 1999, pp. 1013-1020
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOCONJUGATE CHEMISTRY
ISSN journal
10431802 → ACNP
Volume
10
Issue
6
Year of publication
1999
Pages
1013 - 1020
Database
ISI
SICI code
1043-1802(199911/12)10:6<1013:PODCH(>2.0.ZU;2-Z
Abstract
Diaspirin cross-linked hemoglobin (DCLHb), a human hemoglobin that is intra molecularly cross-linked between the a chains (lysine 99(alpha 1)-lysine 99 (alpha 2)), was polymerized with a number of water-soluble, nonimmunogenic polyamide cross-linking agents. The degree of polymerization and the oxygen -carrying capacity depended upon the polyamide reagent, the starting concen tration of DCLHb, the molar ratio of the polyamide reagent to DCLHb used, t he reaction pH, and whether oxy- or deoxy-DCLHb was used in the polymerizat ion reaction.