Effects of environmental level magnetic field exposures on transcription of CMV immediate early promoter DNA in a cell-free in vitro transcription system
H. Miki et al., Effects of environmental level magnetic field exposures on transcription of CMV immediate early promoter DNA in a cell-free in vitro transcription system, BIOELECTROM, 20(8), 1999, pp. 519-521
Effects of environmental levels of magnetic fields (MFs) on RNA synthesis h
ave been investigated by using a cell-free system for in vitro transcriptio
n. Transcription reaction mixtures containing CMV immediate early promoter
DNA plus HeLa cell nuclear extracts were exposed to each of three different
MF field strengths, i.e., 10, 50, and 100 mu T. Each MF exposed extract wa
s paired with a simultaneous sham-exposure control. The present results sho
w no significant differences in amounts of RNA synthesis in extracts of MF
exposed compared with that in the sham controls. This finding is in contras
t to results of prior studies of DNA synthesis in cell-free systems that sh
owed MF exposure effects. The results of the present cell-free system studi
es suggest that the marked differences of MF exposure effects on DNA and on
RNA synthesis direct attention to the complexity involved in confirming si
gnificant effects of exposures to environmental levels of MFs in biosystems
in vivo and in vitro. (C) 1999 Wiley-Liss, Inc.