Surface-based DNA computing operations: DESTROY and READOUT

DNA computing on surfaces is where complex combinatorial mixtures of DNA mo lecules are immobilized on a substrate and subsets are tagged and enzymatic ally modified (DESTROY) in repeated cycles of the DNA computation. A restri ction enzyme has been chosen for the surface DESTROY operation. For the REA DOUT operation, both cycle sequencing and PCR amplification followed by add ressed array hybridization were studied to determine the DNA sequences afte r the computations. (C) 1999 Elsevier Science Ireland Ltd. All rights reser ved.