Facilitating the formation of disulfide bonds in the Escherichia coli periplasm via coexpression of yeast protein disulfide isomerase

Sacchromyces cerevisiae protein disulfide isomerase (yPDI) was expressed in the E. coli periplasm by using plasmids encoding the OmpA-yPDI-(His)(6) fu sion gene under the control of the araBAD, trc, or T7 promoter. The express ion levels of yeast PDI under these promoters were compared. Our results sh owed that yeast PDI expressed into the periplasm could catalyze the formati on of disulfide bonds in alkaline phosphatase, restoring the phoA(+) phenot ype in dsbA(-) mutants. The yeast PDI was purified from the Escherichia col i periplasm and shown to exhibit catalytic properties comparable to those o f the rat enzyme with reduced RNase as substrate. In vivo, coexpression of the yeast PDI increased the yield of bovine pancreatic trypsin inhibitor (B PTI) in E. coli by 2-fold, similar to the effect seen previously with the c oexpression of the rat enzyme. However yeast PDI was more effective than ra t PDI in facilitating the expression of active tissue plasminogen activator (tPA). These results point to differences in the substrate specificity of various PDI enzymes, at least in the context of the E. coli periplasm.