Rice bran lipase: Extraction, activity, and stability

Citation
Av. Prabhu et al., Rice bran lipase: Extraction, activity, and stability, BIOTECH PR, 15(6), 1999, pp. 1083-1089
Citations number
30
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY PROGRESS
ISSN journal
87567938 → ACNP
Volume
15
Issue
6
Year of publication
1999
Pages
1083 - 1089
Database
ISI
SICI code
8756-7938(199911/12)15:6<1083:RBLEAA>2.0.ZU;2-Z
Abstract
A simple procedure for the extraction of the lipolytic activity from rice b ran has been developed. Various conditions of extraction have been optimize d so as to obtain maximum yield of the lipase. It was found that high enzym e activity could be obtained by first defatting the rice bran to remove the lipid component. This was followed by five cycles of aqueous extraction (p otassium phosphate buffer, 50 mM and pH 7, containing 0.5 mM of CaCl2). The stability of the rice bran lipase under storage and operative conditions w as investigated. Further, the influence of glycerol as a stabilizer has bee n assessed. It was found that further purification using micro- and ultrafi ltration yielded an enzyme preparation with higher activity and specific ac tivity and better stability.