Jp. De La Cruz et al., Antiplatelet effect of the anaesthetic drug propofol: influence of red blood cells and leucocytes, BR J PHARM, 128(7), 1999, pp. 1538-1544
1 The present study was designed to investigate the mechanism of the antipl
atelet action of the anaesthetic propofol in vitro.
2 Human whole blood was incubated with different concentrations of propofol
and its solvent Intralipid(R) We determined, platelet aggregometry in whol
e blood, platelet-enriched plasma (PRP). PRP plus red blood cells (RBC), an
d PRP plus leucocytes (LC); platelet production of thromboxane B-2 (TxB(2))
, ATP release by platelet dense granules, adenosine uptake by RBC, intrapla
telet levels of cyclic adenosine monophosphate (cyclic AMP) and cyclic guan
osine monophosphate (cyclic GMP), and LC production of nitric oxide (NO).
3 Propofol-induced inhibition of platelet aggregation was greater in whole
blood (IC50 80-136 mu M) than in PRP (IC50 > 600 mu M), except when aggrega
tion was induced by arachidonic acid, in which case the antiaggregatory eff
ect of the anaesthetic was similar in both media (IC50 72-85 mu M). Inhibit
ion of platelet aggregation correlated significantly with inhibition of TxB
(2) synthesis (r(2) = 0.83). Propofol also inhibited granular ATP release;
this effect was greatest in whole blood.
4 The presence of RBC or LC increased the antiaggregatory effect of propofo
l, mainly when agent. Intralipid inhibited the uptake of adenosine by RBC,
collagen was used as aggregating however this effect probably does not cont
ribute significantly to its antiaggregatory effect.
5 The anaesthetic potentiated the NO-cyclic GIMP pathway, mainly by increas
ing the synthesis of NO by LC. Intralipid had no effect on the NO-cyclic GM
P pathway in the LC-platelet interaction.
6 Propofol inhibited platelet aggregation in human whole blood, possibly th
rough the sum of the effects of Intralipid on the platelet-RBC interaction
and the increased synthesis of NO by LC in the platelet-LC interaction.