Preparation of biotinylated and FITC-labelled phosphorylcholine poly(acrylamide) derivatives and their application for protein ligand-binding studies

Biotin- and FITC-labelled water-soluble poly(acrylamide) derivatives of pho sphorylcholine were prepared by coupling either maleinylated (a) or perioda te-oxidized (b) L-glyceryl phosphorylcholine to poly(acrylamide-allylamine) copolymer. Biotinylated phosphorylcholine poly(acrylamide) derivatives of both types were tested with Limulus polyphemus C-reactive protein and were used for the study of the phosphorylcholine-binding properties of boar semi nal plasma proteins. Binding sites for phosphorylcholine on the surface of bull sperms were visualized using a FITC-labelled derivative of the ligand.