Three proteins from the chloroplastic outer envelope membrane and four prot
eins from the inner envelope membrane have been identified as components of
the chloroplastic protein import apparatus. Multiple molecular chaperones
and a stromal processing peptidase are also important components of the imp
ort machinery. The interactions of these proteins with each other and with
the precursors destined for transport into chloroplasts are gradually being
described using both biochemical and genetic strategies. Homologs of some
transport components have been identified in cyanobacteria suggesting that
at least some of import machinery was inherited from the cyanobacterial anc
estors that gave rise to chloroplasts.