Progress in identification of plant ion channels and development of electro
physiological analyses in heterologous expression systems and in planta, in
combination with reverse genetic approaches, are providing the possibility
of associating molecular entities with physiological functions. Recently,
the first attempts to determine in vivo functions using knockout mutants de
monstrated the roles of root ion channels. The search for proteins interact
ing with such channels leads to an even more complex view of the concerted
action in protein networks.