A. Fogli et al., Identification of two paralogous regions mapping to the short and long arms of human chromosome 2 comprising LIS1 pseudogenes, CYTOG C GEN, 86(3-4), 1999, pp. 225-232
Reiner et al. (1995b) reported on the existence of a gene with a coding reg
ion virtually identical to LIS1, the gene responsible for Miller-Dieker lis
sencephaly. This gene, LIS2, was mapped to chromosome 2p11.2, and a related
pseudogene, LIS2P, was mapped to 2q13-->q14. By sequencing genomic clones
that were mapped by means of 2p and 2q-only hybrids, we now demonstrate the
existence of two LIS1 processed pseudogenes mapping to 2p11.2 and 2q13 (PA
FAH1P1 and PAFAH1P2, respectively). The two sequences appear to lie within
larger paralogous regions and share a 98.6% degree of identity. Comparative
mapping data by cytogenetic analysis on great apes indicate that the dupli
cation of the genomic region comprising the LIS1 pseudogenes occurred in hu
mans. We also demonstrate that the cDNA sequence shown as part of the LIS2
gene and marking its chromosome 2 specificity belongs to the 3' untranslate
d region of a different gene (C1orf6) that we mapped to 1q21 by FISH analys
is.