Interphase FISH for rapid identification of a Down syndrome animal model

Authors
Strovel, J Stamberg, J Yarowsky, PJ
Citation
J. Strovel et al., Interphase FISH for rapid identification of a Down syndrome animal model, CYTOG C GEN, 86(3-4), 1999, pp. 285-287
Citations number
10
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CYTOGENETICS AND CELL GENETICS
ISSN journal
03010171 → ACNP
Volume
86
Issue
3-4
Year of publication
1999
Pages
285 - 287
Database
ISI
SICI code
0301-0171(1999)86:3-4<285:IFFRIO>2.0.ZU;2-M
Abstract
Mouse models of Down syndrome (trisomy 21, DS) have been developed to study the consequences of triplication of regions syntenic to distal human chrom osome 21. We report a technique for the identification of segmental trisomy 16 (Ts65Dn) and diploid progeny at any age using interphase fluorescence i n situ hybridization (FISH) of uncultured tail fibroblasts. Our technique i s faster and less expensive than blood karyotyping.