J. Cansado et al., Presence of non-supressive, M2-related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations, FEMS MICROB, 181(2), 1999, pp. 211-215
Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisi
ae isolated from spontaneous fermentations revealed the presence of a novel
dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, togethe
r with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same ki
nd of viral particles as L and M2 dsRNA. Northern blot hybridization experi
ments indicated that NS dsRNA was derived from M2 dsRNA, likely by deletion
of the internal A + U-rich region. However, unlike S dsRNAs (suppressive f
orms derived from M1 dsRNA in K1 killers), NS dsRNA did not induce exclusio
n of the parental M2 dsRNA when the host strain was maintained for up to 18
0 generations of growth. (C) 1999 Federation of European Microbiological So
cieties. Published by Elsevier Science B.V. All rights reserved.