Presence of non-supressive, M2-related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations

Citation
J. Cansado et al., Presence of non-supressive, M2-related dsRNAs molecules in Saccharomyces cerevisiae strains isolated from spontaneous fermentations, FEMS MICROB, 181(2), 1999, pp. 211-215
Citations number
21
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097 → ACNP
Volume
181
Issue
2
Year of publication
1999
Pages
211 - 215
Database
ISI
SICI code
0378-1097(199912)181:2<211:PONMDM>2.0.ZU;2-M
Abstract
Total dsRNA extractions in five killer K2 strains of Saccharomyces cerevisi ae isolated from spontaneous fermentations revealed the presence of a novel dsRNA fragment (which we named NS dsRNA) of approximately 1.30 kb, togethe r with L and M2 dsRNAs. NS dsRNA appeared to be encapsidated in the same ki nd of viral particles as L and M2 dsRNA. Northern blot hybridization experi ments indicated that NS dsRNA was derived from M2 dsRNA, likely by deletion of the internal A + U-rich region. However, unlike S dsRNAs (suppressive f orms derived from M1 dsRNA in K1 killers), NS dsRNA did not induce exclusio n of the parental M2 dsRNA when the host strain was maintained for up to 18 0 generations of growth. (C) 1999 Federation of European Microbiological So cieties. Published by Elsevier Science B.V. All rights reserved.