Long-term immune recovery after CD34(+) immunoselected and unselected peripheral blood progenitor cell transplantation: a case-control study

Background and Objectives. CD34(+) stem cell selection induces extensive T- cell depletion as a consequence of ex vivo manipulation. The impact of T-ce ll depletion on long-term immunologic recovery after autologous CD34(+) per ipheral blood progenitor cell transplantation (CD34(+) PBPCT) is not well c haracterized. We compared the long term immunologic recovery in two groups of patients submitted to CD34(+) PBPCT or unselected autologous peripheral blood progenitor cell transplantation (uPBPCT), Design and Methods, Eight patients in both groups were closely matched for diagnosis, age, disease status at transplantation and conditioning regimen and lymphocyte phenotype was prospectively evaluated during long-term post- transplantation follow-up. Results. At a median of 18 months after transplantation, CD3(+) lymphocyte subset remained below the normal range in both groups. CD19(+) B lymphocyte s subset after CD34(+) PBPCT was within the normal range In both groups, CD 4(+) lymphocytes were depressed while the CD8(+) lymphocyte subset was incr eased in group A and in the normal range in group B, As a result, inversion of CD4/CD8 ratio was documented in both groups. T-activated lymphocytes (C D3DR(+)) and natural killer (CD16/56(+)) cells were increased In both group s, Interpretation and Conclusions, Long-term immune recovery appears to be una ffected by extensive ex vivo manipulation in this adult population when com pared to recovery after unmanipulated PBPCT, CD34(+) selection, although ca uses an extensive depletion of T lymphocytes in the graft does not represen t a risk factor for delayed CD4(+) recovery late after transplantation. Ele vated numbers of Nh cells and activated T-cells, which have antineoplastic activity, are maintained late after autologous CD34(+) transplantation, (C) 1999, Ferrata Storti Foundation.