SELECTION OF EUROPEAN UNION STANDARD REFERENCE SERA FOR USE IN THE SEROLOGICAL DIAGNOSIS OF INFECTIOUS BOVINE-RHINOTRACHEITIS

Citation
B. Perrin et al., SELECTION OF EUROPEAN UNION STANDARD REFERENCE SERA FOR USE IN THE SEROLOGICAL DIAGNOSIS OF INFECTIOUS BOVINE-RHINOTRACHEITIS, Revue scientifique et technique - Office international des epizooties, 13(3), 1994, pp. 947-960
Citations number
NO
Categorie Soggetti
Veterinary Sciences
ISSN journal
02531933
Volume
13
Issue
3
Year of publication
1994
Pages
947 - 960
Database
ISI
SICI code
0253-1933(1994)13:3<947:SOEUSR>2.0.ZU;2-T
Abstract
A study, consisting of three trials, was undertaken in order to find s tandard reference sera for use in serological diagnostic procedures fo r infectious bovine rhinotracheitis (IBR) in the European Union (EU). A total of forty-nine laboratories participated in the trials, in whic h panels of positive, weak positive, and negative candidate sera were tested blind by neutralisation, indirect and blocking enzyme-linked im munosorbent assay, indirect immunofluorescence and passive haemaggluti nation. A serum which scored positive in all tests in all laboratories was selected as a strong positive standard serum. The chosen negative standard serum gave negative results in virtually all tests. A 1/36 d ilution of a positive serum was chosen as the weak positive standard s erum and only very sensitive techniques were able to score it positive . Three EU reference sera are now available for the standardisation of serological tests for IBR.