B. Perrin et al., SELECTION OF EUROPEAN UNION STANDARD REFERENCE SERA FOR USE IN THE SEROLOGICAL DIAGNOSIS OF INFECTIOUS BOVINE-RHINOTRACHEITIS, Revue scientifique et technique - Office international des epizooties, 13(3), 1994, pp. 947-960
A study, consisting of three trials, was undertaken in order to find s
tandard reference sera for use in serological diagnostic procedures fo
r infectious bovine rhinotracheitis (IBR) in the European Union (EU).
A total of forty-nine laboratories participated in the trials, in whic
h panels of positive, weak positive, and negative candidate sera were
tested blind by neutralisation, indirect and blocking enzyme-linked im
munosorbent assay, indirect immunofluorescence and passive haemaggluti
nation. A serum which scored positive in all tests in all laboratories
was selected as a strong positive standard serum. The chosen negative
standard serum gave negative results in virtually all tests. A 1/36 d
ilution of a positive serum was chosen as the weak positive standard s
erum and only very sensitive techniques were able to score it positive
. Three EU reference sera are now available for the standardisation of
serological tests for IBR.