Examination of signal transduction pathway of stimulated B-1 and B-2 kininreceptors; MAP kinase pathway to AP-1 translocation in HEK 293 cells

B-1 or B-2 kinin receptor-overexpressing HEK293 cells were stimulated with des-Arg(9)-BK or BK, respectively. Each agonist induced translocation of AP -1 into the nuclear fraction as well as activation of MAP kinases in each c ells. MAP kinase inhibitor PD98059 suppressed translocation of AP-1 and ago nist-induced MAP kinase activation in both cells. These results indicate th at stimulation of B-1 or B-2 receptor expresses a feature of the signal tra nsduction pathway of MAP kinase activation to translocation of AP-1. This s ignal transduction pathway of HEK cells through B-1 and B-2 receptors may b e similar in response to respective agonists. (C) 1999 Elsevier Science B.V . All rights reserved.