In situ H-1 NMR study of cytochrome P450 inhibition by metyrapol in mycobacterium aurum MO1

Authors
Combourieu, B Besse, P Sancelme, M Veschambre, H Delort, AM
Citation
B. Combourieu et al., In situ H-1 NMR study of cytochrome P450 inhibition by metyrapol in mycobacterium aurum MO1, J CHIM PHYS, 96(9-10), 1999, pp. 1624-1628
Citations number
13
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE → ACNP
Volume
96
Issue
9-10
Year of publication
1999
Pages
1624 - 1628
Database
ISI
SICI code
Abstract
In situ H-1 NMR was used to monitor incubations of Mycobacterium aurum MO1 with thiomorpholine or morpholine in the presence of metyrapone, a specific inhibitor of P450. Unexpected signals were detected and assigned to metyra pol, the reduction product of metyrapone. The inhibitory effects of racemic metyrapol and of its (-)-enantiomer were compared to that of metyrapone. T hey are similar.