Cr. Beuzon et al., IDENTIFICATION OF SALMONELLA-ABORTUSOVIS BY PCR AMPLIFICATION OF A SEROVAR-SPECIFIC IS200 ELEMENT, Applied and environmental microbiology, 63(5), 1997, pp. 2082-2085
Field and collection isolates of Salmonella abortusovis carry one IS20
0 element in a distinct chromosome location, IS200 is not found in the
corresponding region of the chromosome of other Salmonella serovars.
Sequencing of the boundaries of the S. abortusovis-specific IS200 inse
rtion permitted the design of primers for the amplification of this IS
200 element by PCR. Isolates of S. abortusovis are identified by the a
mplification of a DNA fragment of about 900 bp or larger, PCR amplific
ation of DNA from salmonellae other than S. abortusovis yields either
a fragment of about 200 bp or no product, The high specificity of the
assay is confirmed by the absence of cross-reactivity with the followi
ng templates: (i) sheep DNA, (ii) DNAs from abortion-causing agents ot
her than S. abortusovis, and (iii) DNAs from microorganisms that do no
t cause abortion but are common in flocks.