TRANSFORMATION OF ESCHERICHIA-COLI WITH A LARGE PLASMID OF ACIDIPHILIUM-MULTIVORUM AIU-301 ENCODING ARSENIC RESISTANCE

Authors
SUZUKI K WAKAO N SAKURAI Y KIMURA T SAKKA K OHMIYA K
Citation
K. Suzuki et al., TRANSFORMATION OF ESCHERICHIA-COLI WITH A LARGE PLASMID OF ACIDIPHILIUM-MULTIVORUM AIU-301 ENCODING ARSENIC RESISTANCE, Applied and environmental microbiology, 63(5), 1997, pp. 2089-2091
Citations number
28
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
5
Year of publication
1997
Pages
2089 - 2091
Database
ISI
SICI code
0099-2240(1997)63:5<2089:TOEWAL>2.0.ZU;2-N
Abstract
Acidiphilium multivorum AIU 301 isolated from acid mineral water had s trong arsenic resistance. This bacterium harbored a number of plasmids with different molecular sizes. A plasmid of 56 kbp, named pKW301, wa s isolated from A. multivorum AIU 301. When pKW301 was transferred int o Escherichia coli JM109 by electroporation, an E. coli transformant c arrying pKW301 exhibited resistance to sodium arsenite, sodium arsenat e, and mercuric(II) chloride.