K. Suzuki et al., TRANSFORMATION OF ESCHERICHIA-COLI WITH A LARGE PLASMID OF ACIDIPHILIUM-MULTIVORUM AIU-301 ENCODING ARSENIC RESISTANCE, Applied and environmental microbiology, 63(5), 1997, pp. 2089-2091
Acidiphilium multivorum AIU 301 isolated from acid mineral water had s
trong arsenic resistance. This bacterium harbored a number of plasmids
with different molecular sizes. A plasmid of 56 kbp, named pKW301, wa
s isolated from A. multivorum AIU 301. When pKW301 was transferred int
o Escherichia coli JM109 by electroporation, an E. coli transformant c
arrying pKW301 exhibited resistance to sodium arsenite, sodium arsenat
e, and mercuric(II) chloride.