Application of mechanical strain to neonatal rat ventricular myocytes in cu
lture evokes changes in gene expression reminiscent of those that occur wit
h hypertrophy in vivo, such as stimulation of brain natriuretic peptide (BN
P) gene expression Here, we show that a major component of strain-dependent
BNP promoter activation results from stimulation of p38 mitogen-activated
protein kinase (MAPK) in the cardiac myocyte. Strain increased p38 activity
in a time-dependent fashion. The p38 inhibitor SB203580 led to a reduction
of approximately 60% in strain-activated human BNP (hBNP) promoter activit
y. Cotransfection of wild-type p38 increased both basal and strain-dependen
t promoter activity, while cotransfection with MKK6AL, a dominant-negative
inhibitor of p38 MAPK kinase, resulted in partial inhibition of either p38-
or strain-activated hBNP promoter activity. p38 MAPK increased hBNP promot
er activity through activation of the transcription factor NF-kappa B. Acti
vation of the hBNP promoter by either p38 or strain was mediated by DNA ele
ments present in the 5' flanking sequence of the gene. Mechanical strain pr
omoted assembly of NF-kappa B components on these DNA elements in vitro. Th
us, induction of the hBNP promoter by mechanical strain depends, at least i
n part, on stimulation of p38 and subsequent activation of NF-kappa B. This
activation may play an important role in signaling the increased BNP gene
expression that accompanies hemodynamic overload and cardiac hypertrophy in
vivo.