Jd. Brennan, Preparation and entrapment of fluorescently labeled proteins for the development of reagentless optical biosensors, J FLUORESC, 9(4), 1999, pp. 295-312
A new class of reagentless optical biosensors are emerging based on the use
of engineered proteins that are site-selectively labelled with a fluoresce
nt reporter group. Such sensors can operate either by direct interaction of
the analyte with the bound probe, or by generating a fluorescence response
to an analyte induced conformational change in the protein. In this review
, the preparation, characterization and use of fluorescently labelled prote
ins for the development of reagentless optical biosensors is described. Rec
ent work involving the rational design of fluorescent protein biosensors, a
nd efforts at characterizing the origins of responses from these systems is
highlighted. An overview of the recent and potential future trends in this
area is also given. Finally, methods of immobilizing such biomolecules for
the development of reuseable sensors are described.