Oil-in-water liposomal emulsions: Characterization and potential use in vaccine delivery

Emulsification of mineral oil by phospholipids donated by liposomes compose d of dimyristoyl phosphatidylcholine, dimyristoyl phosphatidylglycerol, cho lesterol, and lipid A by extrusion resulted in the formation of oil-in-wate r liposomal emulsions containing a substantial number of intact liposomes. Increasing the proportion of liposomes from 25 mM to 150 mM phospholipid an d increasing the oil content from 2.5% (v/v) to 42.5% (v/v) changed the flo w characteristics of the emulsions from fluid liquidlike to viscous. Likewi se, the degree of stability of the emulsions was liposomal phospholipid con centration-dependent, ranging from partial emulsification in the range 25-1 00 mM to complete stabilization in the range 125-150 mM. Despite some loss of liposome integrity, as evidenced by the release of liposomal trapped glu cose, emulsification of liposomes containing encapsulated prostate-specific antigen (PSA) exhibited antigen-specific immunostimulation in mice. These results suggest that liposomes containing encapsulated antigen can serve as constituents for the formulation of oil-in-water vaccines.