The electrogenerated chemiluminescent behavior of hemin and its catalytic activity for the electrogenerated chemiluminescence of lucigenin

The electrogenerated chemiluminescent (ECL) behavior of hemin at a platinum electrode in the alkaline solution has been investigated in detail. Under the optimum conditions the linear response range of hemin is 1.0 x 10 (- 5) -1.0 x 10 (- 8) g ml (- 1), the detection limit was 1.0 x 10 (- 8) g ml (- 1), and the relative standard derivation for 1 x 10 (- 7) g ml (- 1) hemin was 2.8%. It has been also found that hemin would catalyze the ECL of lucig enin at a platinum electrode in a neutral solution in the presence of hydro gen peroxide, the catalytic ECL intensity was linear with the concentration of hemin in the range of 1.0 x 10 (- 14) -1.0 x 10 (- 10) g ml (- 1). IgG labeled with hemin was used to examine the ECL catalytic activity of hemin after conjugating to protein, and the results showed that hemin retained EC L catalytic activity when conjugated to protein. (C) 2000 Elsevier Science B.V. All rights reserved.