High performance liquid chromatography method for determination of methyl-5-benzoyl-2-benzimidazole carbamate (mebendazole) and its main degradation product in pharmaceutical dosage forms

Methyl-5-benzoyl-2-benzimidazole carbamate (mebendazole) is a drug used as an anthelmintic. A high performance liquid chromatography method has been d eveloped in this study to determine mebendazole and its degradation product in the pharmaceutical dosage forms (tablets and suspension). The expected major degradation product of mebendazole in the dosage forms has been prepa red, and identified as 2-amino-5-benzoylbenzimidazole. The proposed HPLC as say was found to be selective, accurate (% recoveries were in the range of 99.9-100.9) for both, mebendazole and the degradation product, repeatable a nd reproducible (replicate measurements for short and long term measurement s showed % RSD of less than or equal to 1.4). The methodology could be cons idered as a stability indicating method for mebendazole in pharmaceutical d osage forms. (C) 1999 Elsevier Science B.V. All rights reserved.