J. Leinonen et al., Reactivity of 77 antibodies to prostate-specific antigen with isoenzymes and complexes of prostate-specific antigen, TUMOR BIOL, 20, 1999, pp. 28-34
Seventy-seven antibodies submitted to the ISOBM TD-3 PSA Workshop (TD-3.1 a
nd TD-3.2) were characterized by measuring their reactivity with isoenzymes
of free prostate-specific antigen (PSA), PSA complexed to alpha(1)-antichy
motrypsin (PSA-ACT) and al-proteinase inhibitor (PSA-API). Antibodies were
classified into 15 distinct groups according to their reaction profiles wit
h the various isoenzymes. Some antibodies recognizing both free and complex
ed PSA were inaccurate in measuring total PSA. Eight of the 9 free PSA-spec
ific antibodies cross-reacted more with PSA-API than with PSA-ACT, while 1
antibody reacted less with PSA-API than PSAACT. From the panel of antibodie
s 39 reacted with both free and complexed PSA and were classified as total
PSA antibodies.