Presence of the Streptococcus suis suilysin gene and expression of MRP andEF correlates with high virulence in Streptococcus suis type 2 isolates

Citation
Jj. Staats et al., Presence of the Streptococcus suis suilysin gene and expression of MRP andEF correlates with high virulence in Streptococcus suis type 2 isolates, VET MICROB, 70(3-4), 1999, pp. 201-211
Citations number
31
Categorie Soggetti
Veterinary Medicine/Animal Health",Microbiology
Journal title
VETERINARY MICROBIOLOGY
ISSN journal
03781135 → ACNP
Volume
70
Issue
3-4
Year of publication
1999
Pages
201 - 211
Database
ISI
SICI code
0378-1135(199912)70:3-4<201:POTSSS>2.0.ZU;2-U
Abstract
Nineteen Streptococcus suis type 2 isolates that had been analyzed previous ly for hemolysin production, ribotype, and virulence in pigs were examined for presence of the gene coding for suilysin by PCR amplification, and sout hern blot and hybridization techniques. Based on southern blot and hybridiz ation analysis, all isolates tested contained at least a portion of the sui lysin gene. PCR amplification of the entire gene resulted in gene fragments from five of the seven highly virulent isolates and none of the moderately virulent or avirulent isolates. Additional PCR analysis showed that mutati on or deletions at the 5' end of the suilysin gene in the less virulent iso lates prevented amplification of the sly gene fragment from those isolates. The MRP+ (muramidase-released protein) EF+ (extracellular protein) phenoty pe was also expressed by the same five highly virulent/sly+ isolates. (C) 1 999 Elsevier Science B.V. All rights reserved.