Y. Jyo-oshiro et al., Expression of the fibroblast growth factor receptor 1-4 genes in glomeruliin anti-Thy1.1 mesangial proliferative glomerulonephritis, VIRCHOWS AR, 435(5), 1999, pp. 501-508
Citations number
32
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY
Basic fibroblast growth factor (FGF2) is generally known to induce prolifer
ation of cultured mesangial cells and is expressed in proliferative mesangi
al cells in anti-Thy1.1 mesangial proliferative glomerulonephritis (anti-Th
y1.1 GN). The distribution of the FGF receptor (FGFR) has not been studied
in anti-Thy1.1 GN, so we used in situ hybridization to determine whether ce
lls expressing FGFR1-4 mRNAs could be detected. In normal rats, all glomeru
li were negative for FGFR1-4 mRNA, but those of the mesangial proliferative
phase expressed FGFR1-4 mRNA in proliferative mesangial cells. Proliferati
on of mesangial cells has not been observed in normal rats injected with FG
F2 but it has been noted in anti-Thy1.1 rats injected with FGF2. These data
and our results demonstrate that mesangial cells produce and release FGF2
after injury and that during the proliferative phase these cells upregulate
FGFR in vivo. This study is the first to demonstrate expression of FGFR1-4
mRNAs in pathological glomeruli of anti-Thy1.1 GN. The FGF2 and FGFR1-4 ge
nes were expressed in the proliferative mesangial cells. Upregulation of FG
FR is necessary for mesangial proliferation by FGF2.