Expression of the fibroblast growth factor receptor 1-4 genes in glomeruliin anti-Thy1.1 mesangial proliferative glomerulonephritis

Citation
Y. Jyo-oshiro et al., Expression of the fibroblast growth factor receptor 1-4 genes in glomeruliin anti-Thy1.1 mesangial proliferative glomerulonephritis, VIRCHOWS AR, 435(5), 1999, pp. 501-508
Citations number
32
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY
ISSN journal
09456317 → ACNP
Volume
435
Issue
5
Year of publication
1999
Pages
501 - 508
Database
ISI
SICI code
0945-6317(199911)435:5<501:EOTFGF>2.0.ZU;2-Z
Abstract
Basic fibroblast growth factor (FGF2) is generally known to induce prolifer ation of cultured mesangial cells and is expressed in proliferative mesangi al cells in anti-Thy1.1 mesangial proliferative glomerulonephritis (anti-Th y1.1 GN). The distribution of the FGF receptor (FGFR) has not been studied in anti-Thy1.1 GN, so we used in situ hybridization to determine whether ce lls expressing FGFR1-4 mRNAs could be detected. In normal rats, all glomeru li were negative for FGFR1-4 mRNA, but those of the mesangial proliferative phase expressed FGFR1-4 mRNA in proliferative mesangial cells. Proliferati on of mesangial cells has not been observed in normal rats injected with FG F2 but it has been noted in anti-Thy1.1 rats injected with FGF2. These data and our results demonstrate that mesangial cells produce and release FGF2 after injury and that during the proliferative phase these cells upregulate FGFR in vivo. This study is the first to demonstrate expression of FGFR1-4 mRNAs in pathological glomeruli of anti-Thy1.1 GN. The FGF2 and FGFR1-4 ge nes were expressed in the proliferative mesangial cells. Upregulation of FG FR is necessary for mesangial proliferation by FGF2.