Differential patterns of interaction between HIV type 1 and HTLV type I inmonocyte-derived macrophages cultured in vitro: Implications for in vivo coinfection with HIV type 1 and HTLV type I
J. Szabo et al., Differential patterns of interaction between HIV type 1 and HTLV type I inmonocyte-derived macrophages cultured in vitro: Implications for in vivo coinfection with HIV type 1 and HTLV type I, AIDS RES H, 15(18), 1999, pp. 1653-1666
The interaction between human immunodeficiency virus type 1 (HIV-1) and hum
an T cell leukemia-lymphoma virus type I(HTLV-I) has generated substantial
interest. However, there is disagreement on the in vivo consequences of the
double infection. We investigated the interactions between HIV-1 and HTLV-
I in monocyte-derived macrophages cultured in vitro. For study, the T cell-
tropic strain IIIB and the macrophage-tropic strain Ada-M of HIV-1 were use
d. The HTLV-I was prepared from the supernatants of the virus-producing MT-
2 cell line, We found that coinfection of macrophages with T cell-tropic HI
V-1 and HTLV-I significantly enhanced HIV-1 replication, whereas double inf
ection of the cells with macrophage-tropic HIV-1 and HTLV-I resulted in mar
ked upregulation of HTLV-I production. Stimulatory interactions between HIV
-1 and HTLV-I were mediated by their trans-acting proteins. Results of stud
y on nuclear translocation of proviral DNA showed that the tax gene product
of HTLV-I was able to facilitate the nuclear import of the reverse-transcr
ibed HIV-1(IIIB) DNA, In contrast, the HIV-1 Tat protein did not increase t
he intranuclear trafficking of HTLV-I DNA, which suggests another mechanism
for HTLV-I enhancement by the tat gene product, In conclusion, this study
provides possible mechanisms whereby coinfection of an individual with HIV-
1 and HTLV-I may influence the clinical outcome of double infection.