Ca. Moore et al., Rainbow trout (Oncorhynchus mykiss) possess two somatostatin mRNAs that are differentially expressed, AM J P-REG, 277(6), 1999, pp. R1553-R1561
Citations number
40
Categorie Soggetti
Physiology
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
Previously, we isolated a 624-bp cDNA encoding for a 115-amino acid prepros
omatostatin containing [Tyr(7),Gly(10)]-somatostatin (SS)-14 (now designate
d PPSS-II') obtained from the endocrine pancreas (Brockmann bodies) of rain
bow trout. In this study we have characterized a second cDNA obtained from
trout pancreas that is 600-bp in length and encodes for a 111-amino acid pr
ecursor containing [Tyr(7),Gly(10)]-SS-14 (PPSS-II "). The nucleotide and a
mino acid identity between the two cDNAs is 82.3 and 80.5%, respectively. B
oth PPSS-II' and PPSS-II " mRNA were present in esophagus, pyloric ceca, st
omach, upper and lower intestine, and pancreas, whereas only SS-II " mRNA w
as present in brain. PPSS-II " mRNA was more abundant than PPSS-II' mRNA in
pancreas, whereas PPSS-II' mRNA was more abundant than PPSS-II " mRNA in s
tomach. Fasting increased pancreatic PPSS-II " mRNA levels but had no effec
t on the levels of PPSS-II' mRNA. These results indicate the existence of t
wo nonallelic pancreatic SS-II genes that are differentially expressed, bot
h in terms of distribution among tissues and in terms of relative abundance
within the tissues.