Visualized single cell dynamics and analysis of molecular tricks

The dynamic behaviours of various cells were analysed by video-microscopes and mass spectrometers at the single-cell level. By the video image analysi s of a single cell, real dynamic moments of cell behaviour, which were prev iously accessible only to the imagination, can be visualized - such as the popping of micro-granules, and the exocytosis of neutrophils; a mast cell m odel cell line, RBL-2H3 cells, and pancreatic beta cell model cell line, MI N-6. In combination with the observations of behaviour, more direct molecul ar analyses become much more important for showing the molecular interplay in a cell. The combination of mass spectrometries was applied to analysis a t the single cellular level. Based on the current progress in these analyse s, the future trends in analyses of single cellular dynamism are predicted. (C) 1999 Elsevier Science B.V. All rights reserved.