Batch-type detection cell using a peroxyoxalate chemiluminescence system for capillary electrophoresis

A batch-type chemiluminescence detection cell using a peroxyoxalate system was easily combined with capillary electrophoresis equipment; the device wa s operated without any tedious procedures. Peroxyoxalate reagents and hydro gen peroxide were examined in detail concerning their usage and concentrati on with the cell. Fluorescence-labeled alpha-amino acids showed the detecti on limits of 10 - 100 nM order (S/N=3), which were approximately 2 or 3 ord ers of magnitude lower than those obtained with the absorbance. A mixture s ample of the labeled alpha-amino acids was successfully separated and detec ted.