Probing the radical mechanism of galactose oxidase using an ultrafast radical probe

Authors
Turner, BE Branchaud, BP
Citation
Be. Turner et Bp. Branchaud, Probing the radical mechanism of galactose oxidase using an ultrafast radical probe, BIOORG MED, 9(23), 1999, pp. 3341-3346
Citations number
27
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
9
Issue
23
Year of publication
1999
Pages
3341 - 3346
Database
ISI
SICI code
0960-894X(199912)9:23<3341:PTRMOG>2.0.ZU;2-6
Abstract
Processing of trans-2-phenylcyclopropylmethanols 5 and 6 by the monocopper/ tyrosine radical enzyme galactose oxidase led to mechanism-based inactivati on with a partition ratio, (k(cat) + k(inact))/k(inact), of approximately 1 and a primary deuterium isotope effect, k(inact(H))/k(inact(D)), of 3.2 Th e data are consistent with a radical mechanism for galactose oxidase with a short lived ketyl radical anion intermediate. (C) 1999 Elsevier Science Lt d. All rights reserved.