Aptamers containing thymidine 3 '-O-phosphorodithioates: Synthesis and binding to nuclear factor-kappa B

Authors
Yang, XB Fennewald, S Luxon, BA Aronson, J Herzog, NK Gorenstein, DG
Citation
Xb. Yang et al., Aptamers containing thymidine 3 '-O-phosphorodithioates: Synthesis and binding to nuclear factor-kappa B, BIOORG MED, 9(23), 1999, pp. 3357-3362
Citations number
27
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
9
Issue
23
Year of publication
1999
Pages
3357 - 3362
Database
ISI
SICI code
0960-894X(199912)9:23<3357:ACT3'S>2.0.ZU;2-W
Abstract
Aptamers targeting NF-kappa B containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Bindi ng affinities to NF-KB varied with the number and positions of the dithioat e backbone substitutions. One of the aptamers showed specific binding to a single NF-KB dimer in cell culture extracts. (C) 1999 Elsevier Science Ltd. Ail rights reserved.